Table 1 Table of cryo-EM derived structures of proteins and macromolecular assemblies in complex with small molecule ligands from Homo sapiens, Caenorhabditis elegans, Gallus gallus, Rattus norvegicus, Plasmodium falciparum, Escherichia coli and Bos taurus [105,117119]
Protein nameSpeciesMW (kDa)SymmetryResolution (Å)EMDB CodeLigandLigand MW (Da)Membrane proteinDetergentGrid preparationConc.VPPReference
HaemoglobinH. sapiens64C23.23488NoNoHoley carbon, Quantifoil R1.2/1.31.5 mg/mlYes[32]
Separase–securinC. elegans170C13.83583NoNoQuantifoil R1.2/1.3, graphene-oxide-covered100 nM[35]
p97H. sapiens540C62.33295UPCDC30245464NoNoHoley carbon, Quantifoil R1.2/1.30.9 mg/ml[86]
Lactate dehydrogenaseG. gallus145D22.88191GSK2837808A650NoNoHoley carbon, Quantifoil R1.2/1.31.5 mg/ml[33]
IDH1H. sapiens93C23.88192NoNoHoley carbon, Quantifoil R1.2/1.32.8 mg/ml[33]
IDH1H. sapiens93C23.88193ML309521NoNoHoley carbon, Quantifoil R1.2/1.32.8 mg/ml[33]
γ-SecretaseH. sapiens170C13.43061YesAmphipolsHoley carbon, Quantifoil Au R1.2/1.34 μM[74]
γ-SecretaseH. sapiens170C14.23237DAPT432YesAmphipolsHoley carbon, Quantifoil Au R1.2/1.34 μM[77]
TRPV1R. norvegicus300C43.28118YesNanodiscHoley carbon, Quantifoil Cu R1.2/1.30.5 mg/ml[95]
TRPV1R. norvegicus300C42.98117RTX/DkTx629/ ∼8000YesNanodiscHoley carbon, Quantifoil Cu R1.2/1.30.5 mg/ml[95]
TRPV1R. norvegicus300C43.48119Capsazepine377YesNanodiscHoley carbon, Quantifoil Cu R1.2/1.30.5 mg/ml[95]
TRPV1R. norvegicus300C43.45778YesAmphipolsHoley carbon, Quantifoil R1.2/1.30.3 mg/ml[94]
TRPV1R. norvegicus300C43.85776RTX/DkTx629/ ∼8000YesAmphipolsHoley carbon, Quantifoil R1.2/1.30.3 mg/ml[94]
TRPV1R. norvegicus300C44.25777Capsaicin305YesAmphipolsHoley carbon, Quantifoil R1.2/1.30.3 mg/ml[94]
RibosomeP. falciparum4200C13.22660Emetine481NoNoQuantifoil R1.2/1.3, thin-carbon-film-covered0.5 mg/ml[97]
RibosomeP. falciparum4200C13.28576Mefloquine378NoNoQuantifoil R1.2/1.3, thin-carbon-film-covered∼160 nM[98]
RibosomeE. coli2800C12.92847Kirromycin797NoNoQuantifoil R3.5/1, thin-carbon-film-covered0.07 μM[117]
RibosomeH. sapiens4300C13.64070Cycloheximide281NoNoQuantifoil R2/2, thin-carbon-film-covered0.5 mg/ml[118]
RibosomeE. coli2800C13.68237Avilamycin C1406NoNoQuantifoil R3/3, thin-carbon-film-covered[119]
RibosomeE. coli2800C13.98238Evernimycin1630NoNoQuantifoil R3/3, thin-carbon-film-covered[119]
26S proteasomeH. sapiens1500C13.84146Oprozomib533NoNoQuantifoil R3.5/1, thin-carbon-film-covered[105]
20S proteasomeP. falciparum760C23.63231WLW-vs691NoNoQuantifoil R1.2/1.3, thin-carbon-film-covered0.1 mg/ml[104]
APC/C–TAMEH. sapiens1200C14.03390TAME379NoNoQuantifoil R2/2, thin-carbon-film-covered0.15 mg/ml[106]
Class B GPCRH. sapiens150C14.28623Calcitonin3432YesDetergent MNGHoley carbon, Quantifoil R1.2/1.30.3 mg/mlYes[61]
ABCG2 transporterH. sapiens250C23.83654--YesNanodiscHoley carbon, Quantifoil0.2 mg/ml[108]
Glutamate dehydrogenaseB. taurus334D31.88194NoNoHoley carbon, Quantifoil R1.2/1.33 mg/ml[33]
β-GalactosidaseE. coli465D22.22984PETG300NoNoHoley carbon, Quantifoil R1.2/1.32.3 mg/ml[87]
  • MW, molecular weight; EMDB, Electron Microscopy Data Bank; Conc., concentration; VPP, Volta phase plate.